MRG15 antibody (pAb)

Aliases: MORF4L1, Eaf3, MEAF3, FWP006, S863-6, HsT17725, MORFRG15
Catalog No: 39361 Format: 200 µl $435 Buy
Catalog No: 39362 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
39 kDa
Reactivity:
Human, Other (Wide Range)

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:250 - 1:1,000 dilution

Immunogen

This MRG15 antibody was raised against a peptide corresponding to the C-terminus of human MRG15.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
MRG15 antibody (pAb) tested by Western blot.

MRG15 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) was probed with MRG15 pAb (1:500 dilution).

Background

MRG15 (MORF4-Related Gene on chromosome 15) is the homolog of the yeast Eaf3 protein and belongs to the MRG family of genes, which are involved in various cellular processes as cellular aging, positive and negative transcriptional regulation and DNA damage repair. MRG15 can be associated with both histone deacetylase and histone acetyltransferase complexes (such as the NuA4 histone acetyltransferase complex, which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal Histone H2A and Histone H4). MRG15, thus, potentially plays a role in both transcriptional silencing and activation. In addition to the more conserved MRG motifs, MRG15 also contains a chromodomain near the N-terminus.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for MRG15 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting