CHD2 antibody (pAb)
|Catalog No: 39363||Format: 200 µl||$460||Buy|
|Catalog No: 39364||Format: 10 µl||$115||Buy|
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Applications Validated by Active Motif:
WB: 1:250 - 1:1,000 dilution
The addition of 0.1% Tween 20 in all blocking solutions may reduce background. Individual optimization may be required.
This CHD2 antibody (pAb) was raised against a peptide corresponding to the C-terminus of human CHD2.
Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 is required for normal development of mouse embryos.
Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for CHD2 antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting