CHD2 antibody (pAb)

RRID: AB_2793228
Aliases: EEOC
Catalog No: 39363 Format: 200 µl $460 Buy
Catalog No: 39364 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
260 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:250 - 1:1,000 dilution

The addition of 0.1% Tween 20 in all blocking solutions may reduce background. Individual optimization may be required.

Immunogen

This CHD2 antibody (pAb) was raised against a peptide corresponding to the C-terminus of human CHD2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

CHD2 pAb tested by Western blot.
Detection of CHD2 by Western blot analysis. Nuclear extract of HEK293 cells (25 µg) was probed with CHD2 pAb at a 1:250 dilution.

Background

CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template. CHD2 is required for normal development of mouse embryos.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for CHD2 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting