CHD1 antibody (pAb)

Catalog No: 39729 Format: 100 µl $435 Buy
Catalog No: 39730 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
220 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications: see references

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This CHD1 antibody was raised against a recombinant protein corresponding to amino acids 251-467 of human CHD1.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
CHD1 antibody (pAb) tested by Western blot.

CHD1 antibody (pAb) tested by Western blot.
Detection of CHD1 by Western blot analysis. Lane 1: Nuclear extract of HeLa cells (50 µg). Lane 2: Whole cell extract of adult mouse brain. Both probed with CHD1 antibody (pAb) at a 1:1,000 dilution.

Background

CHD (Chromo-ATPase/Helicase-DNA-binding) proteins, which are ATP-dependent chromatin remodeling factors implicated in RNA polymerase II transcriptional regulation, are characterized by the presence of a double chromodomains (Chromatin Organization Modifier) and SNF2-related helicase/ATPase domains. The CHD1/CHD2 sub-family of CHD proteins also contain a C-terminal DNA binding domain that recognizes AT-rich sequences. CHD proteins alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

CHD1 has been shown to interact with histone H3 trimethylated at lysine 4 (H3K4Me3) and also is required for the maintenance of stem cell pluripotency.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CHD1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting