Histone H3K27me2me3 antibody (mAb)

RRID: AB_2793247
Clone: 7B11
Catalog No: 39536 Format: 100 µg $435 Buy
Catalog No: 39538 Format: 10 µg $105 Buy

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Antibody Type:
Protein A Chromatography
Molecular Weight:
17 kDa
Human, Wide Range Predicted


Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Validated Applications:
ICC/IF: 0.5 - 2 µg/ml dilution
WB: 0.5 - 2 µg/ml dilution

Published Applications:

This antibody has been published for use in ChIP, but has not been validated by Active Motif for this application. ChIP-validated antibodies are available for this target: Cat. No. 39535.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This Histone H3 di/trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3.


Purified IgG in 10 mM sodium phosphate (pH 7.5), 150 mM NaCl, 30% glycerol, 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an ascites version (Catalog No. 39535) of this antibody is also available.

View our Guide to Histone Modifications and Biological Function.



Histone H3 di/trimethyl Lys27 antibody (mAb) tested by immunofluorescence.
Top: HeLa cells stained with Histone H3 di/trimethyl Lys27 antibody (mAb) at 1:500 dilution (red). Middle: Same cells stained with DAPI (blue). Bottom: Both images merged. Staining was carried out using MAX Stain™ Immunofluorescence Tools.

Histone H3 di/trimethyl Lys27 antibody (mAb) tested by Western blot.
HeLa acid extract (10 µg) probed with Histone H3 di/trimethyl Lys27 antibody (mAb) (1:500).

Histone H3 di/trimethyl Lys27 antibody (mAb) specificity data.
HeLa acid extract (10 µg per lane) probed with Histone H3 di/trimethyl Lys27 antibody (mAb) (1:500) with or without pre-incubation of antibody with 1 µM peptide containing the sequence surrounding either Lys9 or Lys27 of Histone H3.
Lane 1: no peptide Lane 2: unmodified Lys27 Lane 3: monomethyl-Lys27 Lane 4: dimethyl Lys27 Lane 5: trimethyl-Lys27 Lane 6: unmodified Lys9 Lane 7: monomethyl-Lys9 Lane 8: dimethyl-Lys9 Lane 9: trimethyl-Lys9.


Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or H3K27me3) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 di/trimethyl Lys27 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K27me2me3 antibody (mAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting