JunB antibody (pAb)

RRID: AB_2615034
Aliases: AP-1
Catalog No: 39549 Format: 200 µl $445 Buy
Catalog No: 39550 Format: 10 µl $110 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
42 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:2,500 dilution

Individual optimization may be required.

Immunogen

This JunB antibody was raised against a synthetic peptide corresponding to amino acids 28-42 of human JunB.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

JunB pAb tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with JunB pAb at a dilution of 1:1,000.

Background

JunB (Jun B proto-oncogene) is a member of the Jun family and forms a component of the AP-1 transcription factor complex. JunB forms heterodimers and homodimers with other members of the Jun/Fos family. It is a hallmark of many lymphomas and stimulates proliferation. Other AP-1 family members consist of c-Fos, Fra-1, Fra-2, FosB, c-Jun, and JunD.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JunB antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting