JunB antibody (pAb)

Aliases: AP-1
Catalog No: 39549 Format: 200 µl $435 Buy
Catalog No: 39550 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
42 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,500 dilution

Individual optimization may be required.

Immunogen

This JunB antibody was raised against a synthetic peptide corresponding to amino acids 28-42 of human JunB.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
JunB antibody (pAb) tested by Western blot.

JunB pAb tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with JunB pAb at a dilution of 1:1,000.

Background

JunB (Jun B proto-oncogene) is a member of the Jun family and forms a component of the AP-1 transcription factor complex. JunB forms heterodimers and homodimers with other members of the Jun/Fos family. It is a hallmark of many lymphomas and stimulates proliferation. Other AP-1 family members consist of c-Fos, Fra-1, Fra-2, FosB, c-Jun, and JunD.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for JunB antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting