RNA pol II CTD phospho Ser2 antibody (pAb)

Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
Catalog No: 39563 Format: 200 µl $435 Buy
Catalog No: 39564 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
240 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 5 - 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Immunogen

This RNA pol II CTD phospho Ser2 antibody was raised against a peptide containing the RNA Pol II CTD sequence phosphorylated at serine 2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
RNA pol II CTD phospho Ser2 antibody (pAb) tested by immunoprecipitation.

RNA Pol II CTD phospho Ser2 antibody (pAb) tested by Immunoprecipitation.
10 µl of RNA Pol II CTD phospho Ser2 antibody was used to immunoprecipitate RNA Pol II CTD phospho Ser2 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the RNA Pol II CTD phospho Ser2 antibody at a dilution of 1:1,000.

RNA pol II CTD phospho Ser2 antibody (pAb) tested by Western blot.

RNA Pol II CTD phospho Ser2 antibody (pAb) tested by Western blot.
HeLa cell nuclear extract (20 µg per lane) was probed with RNA Pol II CTD phospho Ser2 pAb at a dilution of 1:2,000.

RNA pol II CTD phospho Ser2 antibody (pAb) tested by dot blot analysis.

RNA Pol II CTD phospho Ser2 antibody (pAb) tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of RNA Pol II CTD phospho Ser2 pAb. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:2,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: Phospho Ser2 of RNA Pol II CTD peptide.
    Lane 2: Unmodified RNA Pol II CTD peptide.
    Lane 3: Phospho Ser5 of RNA Pol II CTD peptide.

Background

RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for RNA Pol II CTD phospho Ser2 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting