Histone H2A, C-terminal antibody (pAb)

Catalog No: 39591 Format: 200 µl $435 Buy
Catalog No: 39592 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
14 kDa
Reactivity:
Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated

Application Notes


Validated Applications:
IF: 1:500 dilution
WB: 1:500 - 1:1,000 dilution

Published Applications:
WB

Immunogen

This Histone H2A, C-terminal antibody was raised against a peptide containing the C- terminal region of human histone H2A.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Histone H2A, C-terminal antibody (pAb) tested by Western blot.

Histone H2A, C-terminal pAb tested by Western blot.
Detection of Histone H2A by Western blot. The analysis was performed using HeLa acid extract (10 µg, lane 1) and recombinant histone H2A protein (200 ng, lane 2) probed with Histone H2A, C-terminal pAb at a 1:500 dilution).

Histone H2A, C-terminal antibody (pAb) tested by immunofluorescence.

Detection of H2A by immunofluorescence.(/b>
U2OS cells were stained with H2A antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2A antibody staining. Right panel: merge.

Background

Histone H2A is one of the core components of the nucleosome, the basic building block of chromatin. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (Histone H2A, Histone H2B, Histone H3, and Histone H4).

Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H2A, C-terminal antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2A, C-terminal antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting