Histone macroH2A1 antibody (pAb)

Catalog No: 39593 Format: 200 µl $435 Buy
Catalog No: 39594 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
41 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:250 - 1:1,000 dilution
WB: 1:1,000 - 1:2,500 dilution

Published Applications:
WB
IF

Immunogen

This Histone macroH2A1 antibody was raised against recombinant protein corresponding to the N-terminal half of mouse macroH2A1.2, so will react with macroH2A1 and the macroH2A1.1 and macroH2A1.2 isoforms.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Histone macroH2A1 antibody (pAb) tested by immunofluorescence.

Histone macroH2A1 pAb tested by immunofluorescence.
Staining of HeLa cells with Histone macroH2A1 pAb (1:1,000 dilution, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

Histone macroH2A1 antibody (pAb) tested by Western blot.

Histone macroH2A1 pAb tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with Histone macroH2A1 pAb at a dilution of 1:2,000.

Background

Histone macroH2A (mH2A) is a histone variant that has a region that is similar to histone H2A but has a unique C-terminal domain (the macro domain, also called the non-histone domain (NHD)) in addition to the histone-like region. mH2A associates with condensed chromatin, including the inactive mammalian female X chromosome, senescence-associated heterochromatin foci, imprinted genetic loci, and regions of chromatin that are CpG methylated. In vitro assays with mH2A have shown that nucleosomes containing macroH2A are resistant to ATP-dependent chromatin remodeling and transcription factor binding, and that macroH2A is capable of repressing the initiation of RNA pol II catalyzed transcription.

MacroH2A1 consists of two isoforms, macroH2A1.1 and macroH2A1.2, that are produced by alternative splicing; they differ by only 30 amino acids in the C-terminal non-histone domain (NHD).

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone macroH2A1 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone macroH2A1 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting