Aiolos antibody (mAb)

RRID: AB_2615065
Clone: 8B2
Aliases: IKZF3, AIO, ZNFN1A3
Catalog No: 39657 Format: 100 µg $455 Buy
Catalog No: 39658 Format: 10 µg $110 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
70 kDa
Reactivity:
Mouse

Applications

ChIP Validated Western Blot Validated

Application Notes


Published Applications:
ChIP
WB
See references for more information. Individual optimization may be required.

Immunogen

This Aiolos antibody was raised against the N-terminal half of mouse Aiolos protein.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

Aiolos (also known as IKZF3, Ikaros family zinc finger 3 and ZNFN1A3) belongs to a family of zinc-finger proteins that also include Ikaros and Helios. Aiolos is a transcription factor that is important in the regulation of lymphocyte proliferation and differentiation, which is preferentially expressed in peripheral blood leukocytes, spleen and thymus tissue. Both Ikaros and Aiolos can also participate in chromatin remodeling.

Positive Control

This antibody can be used with lymphocytes, spleen, thymus or bone marrow samples.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Aiolos antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting