HIPK2 antibody (mAb)

RRID: AB_2615043
Clone: 3E9
Aliases: PRO0593
Catalog No: 39677 Format: 100 µg $500 Buy
Catalog No: 39678 Format: 10 µg $125 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
130 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IP
ICC/IF
IHC
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This HIPK2 antibody was raised against a recombinant protein including amino acids 898-1051 of mouse HIPK2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

HIPK2 (Homeodomain Interacting Protein Kinase 2) is a serine/threonine kinase present in the nucleus that interacts with homeodomain transcription factors and acts as a co-repressor of transcription. It inhibits cell growth, as well as promotes apoptosis through the phosphorylation and subsequent degradation of the anti-apoptotic factor CTBP1. HIPK2 also phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting