CTBP antibody (pAb)

Aliases: CTBP1, BARS
Catalog No: 39847 Format: 100 µl $435 Buy
Catalog No: 39848 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This CTBP antibody was raised against a peptide within the C-terminal region of human CTBP.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
CTBP antibody (pAb) tested by Western blot.

CTBP antibody (pAb) tested by Western blot.
Nuclear extract of Jurkat cells (30 µg per lane) probed with CTBP antibody (pAb) at a dilution of 1:2,000.

Background

CTBP (C-terminal Binding Protein, CTBP1) is a transcriptional repressor that was originally identified by virtue of its ability to bind the C-terminus of E1A proteins. CTBP serves as a co-repressor for a number of DNA binding transcription factors. CTBP is part of a variety of co-repressor complexes, many of which include HDAC proteins. CTBP also interacts with vertebrate Polycomb proteins including Pc2, suggesting that repression of many CTBP-regulated genes also involves recruitment of the Polycomb complex.

Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for CTBP antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting