SMARCA4 / BRG1 antibody (mAb)

RRID: AB_2614949
Clone: 3G4
Aliases: Brg1, SW1/SNF, HP1-BP72, SNF2beta, b2b692Clo
Catalog No: 39807 Format: 100 µg $445 Buy
Catalog No: 39808 Format: 10 µg $110 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2b
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
180 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ICC/IF

Immunogen

This SMARCA4 / BRG1 antibody was raised against a recombinant protein corresponding to amino acids 214-279 of mouse BRG1.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

Background

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), also known as BRG1, is the central catalytic ATPase subunit of numerous chromatin-remodeling complexes, including SWI/SNF. These complexes are able to use energy from ATP hydrolysis to physically disrupt chromatin architecture of target promoters and facilitate the binding of transcription factors to nucleosomal DNA. SMARCA4 is a co-regulator of transcription and has been implicated in the activation and repression of gene expression through the modulation of chromatin in various tissues and physiological conditions. This protein plays a major role in many cellular processes such as DNA replication, repair and recombination. Mammalian SMARCA4 is usually associated with approximately 10-12 BAF subunits or other proteins involved in regulation of gene expression. SMARCA4 is part of the stem cell-specific BAF complex, esBAF, and is involved in regulating stem cell pluripotency.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting