SMARCA2 / BRM antibody (mAb)

Clone: 1H7A10
Aliases: Snf2l2, brahma, SNF2alpha
Catalog No: 39805 Format: 100 µg $435 Buy
Catalog No: 39806 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
190 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ChIP: 10 µg per ChIP
ICC/IF: 0.5 µg/ml dilution

Published Applications:
ICC/IF
WB
See references for more information. Individual optimization may be required.

Immunogen

This SMARCA2 / BRM antibody was raised against a recombinant protein corresponding to amino acids 48-214 of mouse BRM.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
SMARCA2 / BRM antibody (mAb) tested by ChIP.

SMARCA2 / BRM antibody (mAb) tested by ChIP.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µg of SMARCA2 / BRM antibody or the equivalent amount of rat IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the CD44 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

SMARCA2 / BRM antibody (mAb) tested by immunofluorescence.

SMARCA2 / BRM antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with SMARCA2 / BRM antibody at a 0.5 µg/ml dilution.

Background

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of ATP-dependant chromatin remodeling proteins. SMARCA2 is the catalytic subunit of a multi-subunit protein complex that utilizes the energy from ATP hydrolysis to alter chromatin structure and activate transcription.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SMARCA2 / BRM antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SMARCA2 / BRM antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SMARCA2 / BRM antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting