AIB3 antibody (pAb)

Aliases: Amplified in Breast Cancer 3, Nuclear Receptor Coactivator 6, NCoA6, NRC, NCOA6, ASC2, PRIP, TRBP, RAP250
Catalog No: 39835 Format: 100 µl $435 Buy
Catalog No: 39836 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
250 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunohistochemistry Validated

Application Notes


Published Applications:
IHC
WB
See references for more information. Individual optimization may be required.

Immunogen

This AIB3 antibody was raised against a recombinant protein corresponding to amino acids 22-146 of mouse AIB3.

Buffer

Purified IgG in 70 mM Tris-HCl (pH 8), 105 mM NaCl, 70 mM glycine, 30% glycerol, and 0.035% sodium azide. Also contains BSA. Sodium azide is highly toxic.

References

Background

AIB3 (Amplified in Breast Cancer 3, Nuclear Receptor Coactivator 6, NCoA6) is a chromatin-associated protein that interacts with nuclear hormone receptors (antibodies, kits and proteins) to modify their activity as transcriptional activators. AIB3 / NCoA6 was originally isolated as a ligand-dependent nuclear receptor interacting protein. It is a co-activator that directly binds nuclear receptors and stimulates their transcriptional activity in a hormone-dependent fashion. AIB3 is a multifunctional co-activator necessary for transcriptional activation of a wide spectrum of target genes. AIB3 is involved in growth, development, wound healing and maintenance of energy homeostasis. It can enhance the transcriptional activation of a variety of transcription factors and also associates with a number of important regulators of transcription including CBP and p300. AIB3 / NCoA6 is a component of at least three distinct MLL complexes, suggesting that AIB3 plays a fundamental role in transcriptional activation by modulating chromatin structure through histone methylation. AIB3 may also be involved in the co-activation of the NF-kappa-B pathway. It is amplified and overexpressed in breast, colon and lung cancers. AIB3 function is essential in mammalian development as AIB3 null embryos do not survive. Additionally, AIB null spontaneously undergo apoptosis, indicating that AIB3 has anti-apoptotic and pro-survival functions.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting