AIB1 / SRC-3 antibody (pAb)

Aliases: Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3
Catalog No: 39797 Format: 100 µl $435 Buy
Catalog No: 39798 Format: 10 µl $105 Buy

Request a quote for a bulk order Bulk Order Button


Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
160 kDa
Reactivity:
Human

Applications

ChIP Validated Western Blot Validated

Application Notes


Validated Applications:
ChIP: 10 µl per ChIP
WB: 1:500 - 1:2,000 dilution

Immunogen

This AIB1 antibody was raised against a peptide derived from human AIB1/SRC-3.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
AIB1 / SRC-3 antibody (pAb) tested by ChIP.

AIB1 antibody tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of AIB1 / SRC-3 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the pS2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

AIB1 / SRC-3 antibody (pAb) tested by Western blot.

AIB1 antibody (pAb) tested by Western blot.
Detection of AIB1 by Western blot. Lane 1: HeLa whole-cell extract (30 µg). Lane 2: Whole cell extract (30 µg) of MCF-7 cells. Both probed with AIB1 antibody at a 1:500 dilution.

Background

AIB1 (Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3) is a chromatin-associated protein that interacts with nuclear hormone receptors to modify their activity as transcriptional activators. AIB1 has histone acetyltransferase (HAT) activity and recruits p300/CBP-associated factor (PCAF) and CREB binding protein (CBP) as part of a multisubunit co-activator complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. It is Involved in the coactivation of different nuclear receptors, such as GR, ER, RARs, RXRs, TRs, VDR and PPARs. It is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFκB1 protein.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for AIB1 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AIB1 / SRC-3 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting