CTBP2 antibody (pAb)

Catalog No: 61261 Format: 100 µl $445 Buy
Catalog No: 61262 Format: 10 µl $105 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
50 kDa
Human, Mouse


ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Validated Applications:
ChIP-Seq: 4 µg each
ChIP: 10 µl per ChIP
WB: 1:500 - 1:1,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This CTBP2 antibody was raised against a peptide in the C-terminal region of human CTBP2. This antibody recognizes both isoform 1 and 2.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


ChIP-Seq analysis of CTBP2 (pAb)

CTBP2 (pAb) tested by ChIP-Seq
ChIP was performed using 30 µg of chromatin from the HepG2 cell line and 4 µg of antibody. ChIP DNA was sequenced on the Illumina NextSeq 500 and 12 million sequence tags were mapped to identify CTBP2 binding sites. The image shows binding across a region of chromosome 2. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

CTBP2 antibody (pAb) tested by Western blot.

CTBP2 antibody (pAb) tested by Western blot
Nuclear extract of HeLa cells (30 µg) probed with CTBP2 antibody (1:500).


The CTBP2 (C-terminal binding protein 2) gene encodes a protein that is a corepressor targeting diverse transcription regulators. It functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses. Isoform 2 is also phosphorylated upon DNA damage, probably by ATM or ATR at Thr-179; Ser-181 and Ser-185. Phosphorylation by HIPK2 on Ser-428 induces proteasomal degradation.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CTBP2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with CTBP2 antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting