HDAC1 antibody (mAb)

RRID: AB_2793245
Clone: 10E2
Aliases: HD1, RPD3, GON-10, RPD3L1
Catalog No: 39531 Format: 100 µl $455 Buy
Catalog No: 39532 Format: 10 µl $110 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Culture Supernatant
Host:
Mouse
Molecular Weight:
60 kDa
Reactivity:
Human, Mouse, Rat

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunohistochemistry Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP
IP
IHC
IF


Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Also available as an AbFlex® engineered recombinant antibody.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91215.

Immunogen

This HDAC1 antibody was raised against a KLH-conjugated peptide corresponding to amino acids 467-482 of human and murine HDAC1.

Buffer

Concentrated culture supernatant containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39881) of this antibody that was purified by Protein A Chromatography is also available.

References

 

HDAC1 antibody (mAb) (Clone 10E2) tested by immunofluorescence.
Top left: HeLa cells stained with HDAC1 antibody (mAb) (Clone 10E2) (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.

HDAC1 antibody (mAb) (Clone 10E2) tested by Western blot.
Detection of HDAC1 by Western blot analysis.Nuclear extract of HeLa cells (30μg) was probed with HDAC1 antibody (mAb) (Clone 10E2) at a 1:500 dilution.

Background

HDAC1 (Histone Deacetylase 1, also designated HD1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10), class III (SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT-7) and class IV (HDAC11).

HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the mSin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both Histone H3 and Histone H4 in free histones or nucleosome substrate.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HDAC1 antibody (Clone 10E2).

qPCR Primers

Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with HDAC1 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting