HNF4A antibody (pAb)

Aliases: TCF, HNF4, MODY, MODY1, NR2A1, TCF14, HNF4a7, HNF4a8, HNF4a9, NR2A21, HNF4alpha
Catalog No: 61189 Format: 100 µl $435 Buy
Catalog No: 61190 Format: 10 µl $105 Buy

Request a quote for a bulk order Bulk Order Button

Antibody Type:
Affinity Purified
Molecular Weight:
60 kDa


Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Validated Applications:
ICC/IF: 1:2,000 dilution
WB: 1:500 - 1:2,000 dilution

For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required.


This HNF4A antibody was raised against a peptide derived from the N-terminal region of human HNF4A.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


HNF4A antibody (pAb) tested by immunofluorescence.

HNF4A antibody (pAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with HNF4A antibody at a 1:2,000 dilution.

HNF4A antibody (pAb) tested by Western blot.

HNF4A antibody (pAb) tested by Western blot.
Whole cell extract (20 µg) of Hep G2 cells probed with HNF4A antibody at a dilution of 1:500.


HNF4A (hepatocyte nuclear receptor 4A, NR2A1, TCF14) is an orphan nuclear receptor and activator of transcription. HNF4A is major regulator of the hepatocytic phenotype and regulates genes involved in the control of lipid homeostasis. Mutations in HNF4A can cause maturity onset diabetes of the young (MODY1). HNF4A binds DNA as a homodimer and is involved in the development of the liver, kidney and intestine.

Positive Control

Active Motif's Hep G2 whole-cell extract (Catalog No. 36412) can be used as a positive control for HNF4A antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with HNF4A antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting