HNF-3β / FOXA2 antibody (pAb)

Aliases: TCF3B
Catalog No: 39827 Format: 100 µl $435 Buy
Catalog No: 39828 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Human, Rat

Applications

Western Blot Validated Immunohistochemistry Validated

Application Notes


Validated Applications:
WB: 1:2,000 - 1:5,000 dilution

Published Applications:
IHC
See references for more information. Individual optimization may be required.

Immunogen

This HNF-3β / FOXA2 antibody was raised against a recombinant protein corresponding to amino acids 7-86 of rat HNF-3β /FOXA2.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
HNF-3β / FOXA2 antibody (pAb) tested by Western blot.

HNF-3β / FOXA2 antibody (pAb) tested by Western blot.
Extract of HeLa cells expressing HNF-3β / FOXA2 (50 µg per lane) probed with HNF-3β / FOXA2 antibody (pAb) (1:5,000 dilution).

Background

HNF-3β (Hepatocyte nuclear factor 3 beta, forkhead box A2, FoxA2) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1α, HNF-1β, HNF-3α, HNF-3β, HNF-3γ, HNF-4α, HNF-4γ, HNF-6α and HNF-6β), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3α and HNF-3γ have also been shown to be involved in gastrulation events such as body axis formation.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HNF-3β / FOXA2 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting