EED antibody (mAb)

RRID: AB_2615071
Clone: 41D
Aliases: HEED, WAIT1
Catalog No: 61203 Format: 100 µg $445 Buy
Catalog No: 61204 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
57 kDa
Reactivity:
Human

Applications

ChIP Validated ChIP-Seq Validated Immunohistochemistry Validated

Application Notes


Validated Applications:
ChIP: 4 µl per ChIP
ChIP-Seq & ChIP-chip: 4 µl each
IHC: 2 - 8 µg/ml dilution

Also available as AbFlex™ engineered recombinant antibodies.  For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91135.

Immunogen

This EED antibody was raised against full-length recombinant human EED protein.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

EED antibody (mAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from a human B cell lymphoma cell line (4.5 million cells) and 4 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 32 million sequence tags were mapped to identify EED binding sites. The image on the left shows EED binding across a 1.6 million bp region on chromosome 6. The image on the right shows EED binding at the STX16 start site.

EED mAb tested by Immunohistochemistry .
EED detection by Immunohistochemistry. The analysis was performed using human tonsil paraffin section and the EED mAb.

Background

EED (extraembryonic ectoderm) is a component of the Polycomb group (PcG) multiprotein complex PRC2. The PRC2 complex also contains EZH2 and SUZ12 and is recruited to Polycomb response elements and results in the methylation of histone H3 and lysine 27 and the silencing of gene expression. PRC2 initiates repression of the Hox genes during development and is involved in the maintenance of stem cell pluripotency. EED is a member of the superfamily of WD-40 repeat protein family and is required for PRC2-mediated H3K27 methylation. The PRC2 complex may also recruit platform DNMTs, thereby linking two systems of epigenetic repression.

Learn more about the PRC2 Complex and Stem Cell Identity

qPCR Primers

Active Motif's Human Positive Control Primer Set CCND2 (Cat. No. 71008) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Negative Control Primer Set 1 (Cat. No. 71011) has been validated for both qPCR and endpoint PCR.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with EED antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use EED antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting