RNA Pol II CTD phospho Thr4 antibody (mAb)

RRID: AB_2750848
Clone: 6D7
Aliases: RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220
Catalog No: 61361 Format: 100 µg $455 Buy
Catalog No: 61362 Format: 10 µg $110 Buy
Catalog No: 61961 Format: 50 µg $265 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG2b
Purification:
Protein G Chromatography
Host:
Rat
Molecular Weight:
240 kDa
Reactivity:
Human, Wide Range Predicted

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Dot Blot Validated Enzyme-linked Immunosorbent Assay Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml
DB: 1 µg/ml

Published Applications:
ChIP
IP
ICC/IF
WB
ELISA
See references for more information. Individual optimization may be required.

Immunogen

This antibody was raised against a synthetic peptide corresponding to RNA Pol II CTD phosphorylated at threonine 4.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 

Western blot of RNA pol II CTD phospho Thr4 mAb.
30 µg of HeLa cell nuclear extract was probed with RNA pol II CTD phospho Thr4 antibody at 2 µg/ml in buffer containing 0.05% Tween.

RNA pol II CTD phospho Thr4 antibody tested by bead-based assay
Luminex bead-based specificity analysis was used to confirm the specificity of RNA pol II CTD phospho Thr4 antibody for RNA pol II CTD peptides conjugated to MagPlex Luminex beads and incubated with 50ng of RNA pol II CTD phospho Thr4 antibody. Peptide-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. (Luminex® is a registered trademark of Luminex Corporation.)

Dot blot analysis was used to confirm the specificity of RNA Pol II phospho Thr4 antibody
10, 50, and 250 pmoles of RNA Pol II CTD were spotted onto nitrocellulose and probed with the antibody at 1 µg/ml. Lane 1: RNA Pol II CTD phospho-Thr4. Lane 2: RNA Pol II CTD Thr4 (unmodified)

Background

RNA pol II (RNA polymerase II) is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair. RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with RNA Pol II CTD phospho Thr4 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting