JMJD3 / KDM6B antibody (mAb)

Clone: 67-A2
Catalog No: 61387 Format: 100 µg $435 Buy
Catalog No: 61388 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
150 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

Immunogen

This antibody was raised against a recombinant protein corresponding to amino acids 1028-1684 of human JMJD3 / KDM6B.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 
JMJD3 / KDM6B antibody (mAb) tested by Western blot.

JMJD3/ KDM6B mAb (Clone 67-A2) tested by Western blot.
JMJD3/ KDM6B detection by Western blot. The analysis was performed using cell lysate from BJ cells (lane 1) and BJ cells irradiated (lane 2).

Background

JMJD3 / KMD6B (Jumonji domain-containing protein 3 lysine (K)-specific demethylase 6B) Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting