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Smyd3 antibody (pAb)

Aliases: SET and MYND domain containing 3, KMT3E, ZMYND1, ZNFN3A1, bA74P14.1
Catalog No: 61407 Format: 100 µl $425 Buy Now
Catalog No: 61408 Format: 10 µl $99 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:50 kDa


Western Blot Validated Immunohistochemistry Validated

for Smyd3 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications:

for Smyd3 antibody (pAb)Immunogen

This antibody was raised against a peptide within human Smyd3.

for Smyd3 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

for Smyd3 antibody (pAb)References

Smyd3 antibody (pAb) tested by Western blot.

Smyd3 antibody (pAb) tested by Western blot.
K-562 nuclear extract (30 µg) probed with Smyd3 antibody at a dilution of 1:500.

for Smyd3 antibody (pAb)Background

Smyd3 (SET and MYND domain containing 3) is a histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Recently, Smyd3 was described as the methyltransferase responsible for a novel site of histone modification, H4K5 methylation,

for Smyd3 antibody (pAb)Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for Smyd3 antibody.

for Smyd3 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for Smyd3 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

Smyd3 antibody (pAb)

Data Thumbnails

Smyd3 antibody (pAb) tested by Western blot.

Western blot of Smyd3 pAb.
(Click image to enlarge and see details.)