Smyd3 antibody (pAb)

Aliases: SET and MYND domain containing 3, KMT3E, ZMYND1, ZNFN3A1, bA74P14.1
Catalog No: 61407 Format: 100 µl $435 Buy
Catalog No: 61408 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunohistochemistry Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Published Applications:
IHC (FFPE)

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This antibody was raised against a peptide within human Smyd3.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.

References

 
Smyd3 antibody (pAb) tested by Western blot.

Smyd3 antibody (pAb) tested by Western blot.
K-562 nuclear extract (30 µg) probed with Smyd3 antibody at a dilution of 1:500.

Background

Smyd3 (SET and MYND domain containing 3) is a histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Recently, Smyd3 was described as the methyltransferase responsible for a novel site of histone modification, H4K5 methylation,

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for Smyd3 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting