FTO antibody (pAb)

Aliases: ALKBH9
Catalog No: 61431 Format: 100 µl $435 Buy
Catalog No: 61432 Format: 10 µl $105 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
60 kDa


Western Blot Validated

Application Notes

Validated Applications:
WB: 1:500 - 1:2,000 dilution


This antibody was raised against a peptide within the N-terminal region of human FTO.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide.


FTO antibody (pAb) tested by Western blot.

FTO antibody (pAb) tested by Western blot.
Detection of FTO by Western blot analysis (30 µg per lane). Lane 1: Nuclear extract of Caco-2 cells. Lane 2: Nuclear extract of HEK-293 cells. Both probed with FTO antibody (pAb) at a 1:500 dilution.


FTO (fat mass and obesity associated) is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. This protein has high activity towards single-stranded RNA containing 3-methyluracil and single-stranded DNA containing 3-methylthymine. FTO has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and has no activity towards 1-methylguanine or double-stranded DNA. FTO contributes to the regulation of the global metabolic rate, energy expenditure, energy homeostasis, regulation of body size and body fat accumulation. FTO has recently been shown to have the highest activity towards demethylation of 6-methyladeninine in single-stranded RNA suggesting that FTO is involved in processing of nuclear RNAs and may play an important role in RNA epigenetics.

Positive Control

Active Motif's Caco-2 nuclear extract (Catalog No. 36035) can be used as a positive control for FTO antibody.


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting