BRD3 antibody (pAb)

RRID: AB_2737028
Aliases: ORFX, RING3L
Catalog No: 61489 Format: 100 µl $490 Buy
Catalog No: 61490 Format: 10 µl $120 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
100 kDa
Reactivity:
Human, Mouse

Applications

CUT&RUN Validated ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 10 µl per ChIP
ChIP-Seq & ChIP-chip: 10 µl each
WB: 1:500- 1:2,000 dilution
CUT&RUN: 1 µl per 50 µl reaction

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-qPCR, ChIP-Seq
CUT&RUN
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This antibody was raised against a peptide within the C-terminal region of mouse BRD3.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

BRD3 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human H526 cells and 10 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to show the expected promoter localization.

BRD3 antibody (pAb) tested by ChIP.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human H526 cells and 10 µl BRD3 antibody. ChIP DNA was used in qPCR with the control primer or gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

BRD3 antibody (pAb) tested by Western blot.
Detection of BRD3 by Western blot analysis. Lane 1: Whole cell extract of HeLa cells (20 µg). Lane 2: Whole cell extract of NIH-3T3 cells. Both probed with BRD3 antibody (pAb) at a 1:500 dilution.

BRD3 antibody (pAb) tested by CUT&RUN
CUT&RUN was performed using 500,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NovaSeq. Data was collected from 23 million reads, and BRD3 data is shown for Chromosome 19.

Background

BRD3 (Bromodomain Containing 3) is a member of the mammalian BET (Bromodomain Extra Terminal) family of proteins, binds hyperacetylated chromatin and has been reported to interact with acetylated Histone H4K5, Histone H4K12 and Histone H3K14. It plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with BRD3 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting