BRD3 antibody (pAb)
|Aliases: ORFX, RING3L|
|Catalog No: 61489||Format: 100 µl||$490||Buy|
|Catalog No: 61490||Format: 10 µl||$120||Buy|
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Applications Validated by Active Motif:
ChIP: 10 µl per ChIP
ChIP-Seq & ChIP-chip: 10 µl each
WB: 1:500- 1:2,000 dilution
CUT&RUN: 1 µl per 50 µl reaction
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.
This antibody was raised against a peptide within the C-terminal region of mouse BRD3.
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
BRD3 (Bromodomain Containing 3) is a member of the mammalian BET (Bromodomain Extra Terminal) family of proteins, binds hyperacetylated chromatin and has been reported to interact with acetylated Histone H4K5, Histone H4K12 and Histone H3K14. It plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with BRD3 antibody (pAb).
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting