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JMJD6 antibody (pAb)

Catalog No: 61493 Format: 100 µl $425 Buy Now
Catalog No: 61494 Format: 10 µl $99 Buy Now

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Antibody Type:Polyclonal
Purification:Affinity Purified
Molecular Weight:52 kDa


Western Blot Validated

for JMJD6 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for JMJD6 antibody (pAb)Immunogen

This antibody was raised against a peptide within the N-terminal region of human JMJD6.

for JMJD6 antibody (pAb)Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for JMJD6 antibody (pAb)References

JMJD6 antibody (pAb) tested by Western blot.

JMJD6 antibody (pAb) tested by Western blot.
Nuclear extract of Raji cells (20 µg) probed with JMJD6 antibody (pAb) at a dilution of 1:500.

for JMJD6 antibody (pAb)Background

JMJD6 (Jumonji Domain-Containing Protein 6) is a dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. JMJD6 acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. Required for differentiation of multiple organs during embryogenesis and acts as a key regulator of hematopoietic differentiation. May act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA.

for JMJD6 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for JMJD6 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

JMJD6 antibody (pAb)

Data Thumbnails

JMJD6 antibody (pAb) tested by Western blot.

Western blot of JMJD6 pAb.
(Click image to enlarge and see details.)