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Recombinant NUAK1 (ARK5) protein
| Aliases: SNF1-like kinase 1, OMPHK1, KIAA0537 |
| Catalog No: 81331 | Format: 20 µg | $465 | Buy |
| Catalog No: 81631 | Format: 1 mg | $4,000 | Buy |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
NUAK1, also known as ARK5, is the fifth member of the AMPK catalytic subunit family. It is a serine/ threonine-protein kinase that are activated by phosphorylation at its Ser600 by Akt, which has been reported to be a key player in tumor malignancy. The activated NUAK1 involves in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation an tumor progression, such as it acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of ‘Ser-464’ of LATS1,and Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Which also involves in DNA damage response by phosphorylating p53/ TP53 at ‘Ser-15’ and ‘Ser-392’, and it is a sensitive indicator of cellular energy status and have been found to promote tumor cell survival during nutrient starvation.
Application Notes
Recombinant NUAK1 / ARK5 protein is suitable for use in in enzyme kinetics, inhibitor screening, and selectivity profiling.
Protein Details
Recombinant NUAK1 / ARK5 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_055655.1) with a C-terminal FLAG tag. The molecular weight of the protein is 76 kDa.
Recombinant NUAK1 / ARK5 protein gel
10% SDS-PAGE with Coomassie blue staining
MW: 76 kDa
Purity: >95%
HTRF assay for NUAK1 / ARK5 activity
1 μM STK S3 substrate was incubated with different concentrations of NUAK1 / ARK5 protein in 10 μl reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 μM ATP for 1 hour. The 10 μl detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
