Recombinant CDK1 protein

Aliases: CDC2, CDC28A, P34CDC2, Cell Division Protein Kinase 1, CDKN1, P34 Protein Kinase Cyclin-Dependent Kinase 1
Catalog No: 82082 Format: 20 µg $480 Add to Cart
Catalog No: 82682 Format: 1 mg $4,150 Add to Cart

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Expressed In: Baculovirus Protein Species: Human

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.

Background

CDK1 (Cyclin-dependent kinase 1) , also known as CDC2, CDC28A and P34CDC2. CDK1 plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase cyclins. Associated with its cognate partner cyclin B, is a key enzyme for entry in mitosis and is essential for spindle morphogenesis. CDK1 up-regulates microtubule dynamics when added in cell free Xenopus extracts. CDK1inactivation is necessary for proper anaphase spindle dynamics and for cytokinesis. Large amounts of CDK1 induce the depolymerization of interphase microtubules when injected into mammalian cells and the destabilization of microtubule arrays when added on lysed mammalian cells.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

Protein Details

Recombinant CDK1 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_001777.1) with an C-terminal FLAG tag. The molecular weight of CDK1 is 35.79 kDa.

References

Human

 
Recombinant CDK1 protein gel

Recombinant CDK1 protein gel
10% SDS-PAGE Coomassie staining
MW: 35.79 kDa
Purity: >95%

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt. This product is for research use only and is not for use in diagnostic procedures.