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Recombinant CDK1 / Cyclin B1 Complex
| Catalog No: 82134 | Format: 20 µg | $505 | Add to Cart |
| Catalog No: 82734 | Format: 1 mg | $5,850 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
The CDK1 / Cyclin B1 complex is a master regulator of the G2/M transition and progression through mitosis in eukaryotic cells. As a cyclin-dependent kinase complex, it phosphorylates numerous substrates to drive critical mitotic events, including nuclear envelope breakdown, chromosome condensation, spindle assembly, and activation of the anaphase-promoting complex/cyclosome (APC/C) for sister chromatid separation. Beyond its canonical cell cycle role, CDK1 / Cyclin B1 localizes to mitochondria and phosphorylates complex I proteins to enhance ATP production, thereby coupling energy generation with division demands. It also maintains chromosomal stability by regulating kinetochore microtubule attachments and spindle checkpoint signaling through phosphorylation of Mps1 and Aurora B kinases. In oocyte meiosis, the complex is essential for the metaphase I-to-anaphase I transition by promoting APC/C activation and homologous chromosome segregation. Dysregulation of CDK1 / Cyclin B1 contributes to cancer pathogenesis, as its overexpression or defective function (e.g., impaired Cyclin B1 induction) is linked to tumor proliferation, chemoresistance (e.g., T-DM1 in breast cancer), and genomic instability, making it a significant therapeutic target.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant CDK1 / Cyclin B1 Complex that includes full length human CDK1 protein (accession number NP_001777.1) with an C-terminal His tag and full length human Cyclin B1 protein (accession number NP_114172.1) with an C-terminal FLAG tag was expressed in Hi5 cells. The molecular weights of CDK1 and Cyclin B1 are 35.73 kDa and 50.03 kDa, respectively.
References
Human
Recombinant CDK1/Cyclin B1 protein gel
4%-20%% SDS-PAGE Coomassie staining
MW: CDK1: 35.73 kDa, Cyclin B1:50.03 kDa
Purity: >90%
ADP-Glo assay for CDK1/Cyclin B1 Complex activity
0.1 µg/µl Histone H1 was incubated with different concentrations of CDK1 / Cyclin B1 complex in a 5 µl reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 1 mM DTT, 0.1 mg/ml BSA and 20 µM ATP at 30℃ for 1 hour, then 5 µl ADP-Glo Reagent was added. After 40 minutes of incubation at RT, 10 µl Kinase Detection Reagent was added in the system. Following 30 minutes incubation at RT, luminescence was detected with a plate reader.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt. This product is for research use only and is not for use in diagnostic procedures.
