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Recombinant POLQ (1-894) protein
| Aliases: DNA polymerase theta, POLH |
| Catalog No: 82145 | Format: 20 µg | $470 | Add to Cart |
| Catalog No: 82745 | Format: 1 mg | $4,150 | Add to Cart |
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Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
POLQ, also known as DNA polymerase theta, is a unique and specialized DNA polymerase that plays a critical role in an alternative, error-prone pathway for repairing double-strand breaks, known as theta-mediated end joining (TMEJ). Unlike classical repair mechanisms, TMEJ functions as a backup system to ligate broken DNA ends with minimal homology, often resulting in mutations and deletions. While this promotes genomic instability, it is essential for the survival of cancer cells that have defects in other repair pathways like homologous recombination. Consequently, POLQ is overexpressed in many cancers and has emerged a promising therapeutic target. Its functions also include mitigating replication stress and protecting against DNA end resection.
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
Protein Details
Recombinant POLQ (1-894) protein that includes amino acids 1-894 of human POLQ protein (accession number NP_955452.3) was expressed in a baculovirus expression system and contains an N-terminal FLAG tag. The molecular weight of the protein is 100.95 kDa.
Recombinant POLQ (1-894) protein gel
4-20% SDS-PAGE gel
MW: 100.95
Purity: >95%
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
