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Recombinant RORA protein
| Aliases: RAR related Orphan Receptor Alpha |
| Catalog No: 82159 | Format: 20 µg | $480 | Add to Cart |
| Catalog No: 82759 | Format: 1 mg | $4,150 | Add to Cart |
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Applications
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
References
Human
Recombinant RORA protein gel
4-20% SDS-PAGE gel
MW: 60.25
Purity: >95%
HTRF Assay for Recombinant RORA activity.
1 µM substrate dsDNA (5’ biotin ATATAACTAGGTCATA 3’) was incubated with different concentrations of RORA protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 0.1% BSA at room temperature for 1 hour. Anti-FLAG antibody was used to detect reaction products.
Background
RORA (RAR-related Orphan Receptor Alpha) is a nuclear receptor that functions as a ligand-dependent transcription factor with diverse roles in development and homeostasis. It regulates the expression of genes involved in lipid metabolism, immune function, and the circadian clock, particularly in the brain where it influences neuronal development and Purkinje cell survival. Dysregulation of RORA is implicated in metabolic disorders, certain autoimmune diseases, and neurological conditions such as autism spectrum disorders.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
