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Recombinant NR2C1 protein
| Aliases: Nuclear Receptor Subfamily 2 Group C Member 1 |
| Catalog No: 82161 | Format: 20 µg | $480 | Add to Cart |
| Catalog No: 82761 | Format: 1 mg | $4,150 | Add to Cart |
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Applications
Application Notes
This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for this product is shown.
References
Human
HTRF Assay for Recombinant NR2C1 activity.
1 µM substrate dsDNA (5’ biotin-ATAGGTCACGGAGAGGTCAGC 3’) was incubated with different concentrations of NR2C1 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 100 µM ZnCl2, 0.1% BSA at room temperature for 1 hour. Anti-FLAG antibody was used to detect reaction products.
HTRF Assay for Recombinant NR2C1 activity.
1 µM substrate dsDNA (5’ biotin-ATAGGTCACGGAGAGGTCAGC 3’) was incubated with different concentrations of NR2C1 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.0, 100 µM ZnCl2, 0.1% BSA at room temperature for 1 hour. Anti-FLAG antibody was used to detect reaction products.
Background
NR2C1 (Nuclear Receptor Subfamily 2 Group C Member 1)
, also known as TR2 (Testicular Receptor 2), is a nuclear receptor that functions as a transcriptional repressor or activator in a context-dependent manner. It is involved in embryonic development, cell proliferation, and differentiation, particularly within the hematopoietic and nervous systems, by regulating gene networks related to growth and metabolism. Emerging research also links its dysregulated expression to the development and progression of certain cancers.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
