Recombinant Histone H1.X

Catalog No: 82168 Format: 50 µg $480 Add to Cart
Catalog No: 82768 Format: 1 mg $2,950 Add to Cart

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Applications

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

References

Human

 
Recombinant H1.X protein gel

Recombinant H1.X protein gel
4-20% SDS-PAGE gel
MW: 23.55
Purity: >90%

Background

Histone H1.X, also known by its gene symbol H1FX or alternative names such as H1-10, H1.10, and H1 histone family member X, is a replication-independent subtype of the linker histone H1 family that exhibits distinct biochemical properties and plays specialized roles in chromatin dynamics. Unlike the more abundant somatic H1 variants, H1.X demonstrates significantly lower DNA, nucleosome, and histone chaperone binding activities, which results in its remarkably rapid exchange on and off chromatin compared to other H1 subtypes. This highly dynamic linker histone accumulates in the nucleolus during interphase and localizes to the chromosome periphery during mitosis, where it has been shown to be essential for proper chromosome alignment and segregation. Furthermore, H1.X displays unique chromosomal binding site preferences that differ from other variants like H1.0, with its N-terminal, globular, and C-terminal domains cooperatively determining its specific genomic localization patterns and contributing to its regulatory functions in higher-order chromatin structure.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting