Recombinant SMARCAD1 protein

Aliases: ETL1, ADERM, ATP-Dependent Helicase 1
Catalog No: 82170 Format: 20 µg $480 Add to Cart
Catalog No: 82770 Format: 1 mg $4,150 Add to Cart

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Applications

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product.  All available data for this product is shown. 

References

Human

 
Recombinant SMARCAD1 protein gel

Recombinant SMARCAD1 protein gel
4-20% SDS-PAGE gel
MW:118.7 kDa
Purity: >95%

ADP-Glo Assay for SMARCAD1 activity.

ADP-Glo assay for SMARCAD1 activity 0.4 µM polynucleosomes H3.1 (Cat# 31466) was incubated with different concentrations of SMARCAD1 protein in a 10 µl reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 1 mM DTT, 0.1 mg/ml BSA and 20 µM ATP at 30℃ for 1 hour, then 10 µl ADP Glo Reagent wad added. After 40 minutes incubation at RT, 20 µl Kinase Detection Reagent was added in the system. Following 30 minutes incubation at RT, luminescence was detected with a plate reader.

Background

SMARCAD1 (SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1), also known as ETL1, HEL1, HRZ, or KIAA1122, is a multifunctional ATP-dependent nucleosome remodeling enzyme belonging to the SNF2/RAD54 helicase family . This protein plays a dual role in both the DNA damage response and the maintenance of epigenetic stability; it promotes the resection of DNA ends at double-strand breaks (DSBs) to facilitate homologous recombination repair, while it is also required post-replication to restore heterochromatin organization by directing histone H3/H4 deacetylation and H3K9 trimethylation. SMARCAD1 antagonizes the repair inhibitor 53BP1 at damage sites and colocalizes with PCNA at replication forks to ensure proper chromatin silencing, with mutations in this gene leading to conditions such as adermatoglyphia (absence of fingerprints) and Basan syndrome.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting