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Antibodies

AbFlex® Rad21 antibody (rAb)

Catalog No: 91245 Format: 100 µg $375 Buy Now
Catalog No: 91246 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:100 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Immunofluorescence Validated

for AbFlex Rad21 antibody (rAb)Application Notes


Validated Applications:
IF: 0.25 - 1 µg/ml
WB: 0.5- 2 µg/mL

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 39383.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

for AbFlex Rad21 antibody (rAb)Immunogen

This antibody was raised against recombinant protein corresponding to the N-terminal half of mouse Rad21.

for AbFlex Rad21 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

for AbFlex Rad21 antibody (rAb)References

 
AbFlex<sup>®</sup> Rad21 antibody (rAb) tested by Western blot.

AbFlex® Rad21 antibody (rAb) tested by Western blot.
20 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Rad21 antibody at 1 µg/ml.

AbFlex<sup>®</sup> Rad21 antibody (rAb) tested by immunofluorescence.

AbFlex® Rad21 antibody (rAb) tested by Immunofluorescence.
HeLa cells stained with 0.5 µg /mL of AbFlex Rad21 antibody (rAb) (Top Panel) or DAPI (Lower Panel) followed by anti-mouse IgG-488.

for AbFlex Rad21 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Rad21 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells

Rad21 (HR21, MCD1, NXP1, hHR21) is highly similar to the gene product of S. pombe Rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. Rad21 is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of Rad21 with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with AbFlex® Rad21 antibody (rAb).

for AbFlex Rad21 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Rad21 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Rad21 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Rad21 antibody (rAb) tested by Western blot.

Western blot of AbFlex® Rad21 antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> Rad21 antibody (rAb) tested by immunofluorescence.

Immunofluorescence stain of AbFlex® Rad21 antibody (rAb).
(Click image to enlarge and see details.)