AbFlex® PHD1 (EGLN2) antibody (rAb)

Aliases: prolyl-4-hydroxylase 1, Egl-9 Family Hypoxia Inducible Factor 2, HIF-PH1
Catalog No: 91447 Format: 100 µg $400 Add to Cart
Catalog No: 91448 Format: 10 µg $90 Add to Cart

Request a quote for a bulk order Request Quote

Product Review Submit a Review

Antibody Type:
Recombinant
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
43.65 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunofluorescence Validated

Application Notes

Applications Validated by Active Motif:
WB: 0.1 - 0.5 µg/ml
IF: 0.4 - 2 µg/ml

Immunogen

This EGLN2 (PHD1) antibody was raised against a peptide of human EGLN2 (PHD1).

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
AbFlex<sup>®</sup> PHD1 (EGLN2) antibody (rAb) tested by Western blot.

AbFlex® EGLN2 (PHD1) rAb antibody tested by Western blot.
U2OS, HeLa and A549 whole cell extracts (40 µg) were probed with AbFlex® EGLN2 (PHD1) antibody at 0.2 µg/mL.

AbFlex<sup>®</sup> PHD1 (EGLN2) antibody (rAb) tested by immunofluorescence.

AbFlex® EGLN2 (PHD1) rAb antibody tested by immunofluorescence.
EGLN2 (PHD1) was detected in human U2OS cells using AbFlex® PHD1antibody (rAb) at 2 µg/mL, followed by Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L).​

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag forenzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® EGLN2 (PHD1) antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells. Prolyl Hydroxylase Domain Containing Protein 1 (PHD1) or Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2), also known as HIF-PH1 or EIT6, is a prolyl hydroxylase. PHD1 is a cellular oxygen sensor that catalyzes the post translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins under normoxic conditions. It can hydroxylate a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A, also HIF2A. PHD1 prefers the CODD site for both HIF1A and HIF2A.NODD, and C-terminal, CODD) of HIF1A, also HIF2A.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting