Cart
Products
AbFlex® GLI1 antibody (rAb)
| Aliases: GLI1 family zinc finger 1, PPD1, PAPA8 |
| Catalog No: 91501 | Format: 100 µg | $400 | Add to Cart |
| Catalog No: 91502 | Format: 10 µg | $90 | Add to Cart |
Request a quote for a bulk order Request Quote
Product Review Submit a Review
Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.5 - 2 µg/mL
IP: 2 µg per IP
IF: 2 µg/mL
ChIP-Seq: 2 µg per IP
Immunogen
This GLI1 antibody was raised against a peptide of human GLI1.
Buffer
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
AbFlex® GLI1 rAb antibody tested by Western blot.
40 µg of SJSA-1 whole cell extracts were probed with AbFlex® GLI1 antibody at 0.5 µg/mL.
AbFlex® GLI1 rAb antibody tested by immunoprecipitation.
2 µg of antibody was used to immunoprecipitate GLI1 from 1 mg of SASJ-1 whole cell extract, 40% IP product (Lane 2) was then run on SDS-PAGE and probed with the same antibody at 0.5 µg /ml. Tween-20 at 0.1% was added to blocking buffers to perform Western blot and reduce background. 2 µg of rabbit IgG was used as negative control for IP (Lane 2).
AbFlex® GLI1 rAb antibody tested by immunofluorescence.
GLI1 was detected in human HeLa cells using AbFlex® GLI1 antibody at 2 µg/mL. Followed by Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L).DAPI at 594 nm.
AbFlex® GLI1 rAb antibody tested by ChIP-Seq.
ChIP was performed using ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 18 µg of chromatin from SJSA-1 cells and 2 µg of GLI1 antibody. ChIP DNA was sequenced on the Illumina NovaSeq and 43 million sequence tags were mapped to identify GLI1 binding sites. The image shows binding across a region of chromosome 1.
Background
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex® antibody contains a 6xHis Tag, a Biotinylation Tag forenzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
AbFlex® GLI1 antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells.
GLI1 (GLI family zinc finger 1, also known as PPD1, PAPA8) is a member of the Kruppel family of zinc finger proteins. GLI1 is activated by the sonic hedgehog signal transduction cascade. It plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AbFlex® GLI1 antibody (rAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
