Cart
Products
AbFlex® CCNA2 (Cyclin A2) antibody (rAb)
| Aliases: Cyclin A2, CCN1, CCNA |
| Catalog No: 91531 | Format: 100 µg | $400 | Add to Cart |
| Catalog No: 91532 | Format: 10 µg | $90 | Add to Cart |
Request a quote for a bulk order Request Quote
Product Review Submit a Review
Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.1 – 1 µg/mL
IP: 2 µg per IP
IF: 0.4-2 µg/mL
ChIP-Seq: 2 µg per IP
Immunogen
This CCNA2 (cyclin A2) antibody was raised against a peptide of human CCNA2 (cyclin A2).
Buffer
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
AbFlex® CCNA2 rAb antibody tested by Western blot.
40 µg of K562 (Lane1) and Raji (Lane 2) whole cell extracts were probed with AbFlex® CCNA2 antibody at 0.2 µg/mL.
AbFlex® CCNA2 rAb antibody tested by immunoprecipitation.
2 µg of antibody was used to immunoprecipitate CCNA2 from 1 mg of K562 whole cell extract, 40% of IP product (Lane 3) and 40 µg of Input proteins (Lane 1) was run on an SDS-PAGE and probed with the same antibody at 0.2 µg /ml. Tween-20 at 0.1% was added to blocking buffers to perform Western blot and reduce background. 2 µg of rabbit IgG was used as negative control for IP (Lane 2).
AbFlex® CCNA2 rAb antibody tested by immunofluorescence.
CCNA2 was detected in human HeLa cells using AbFlex CCNA2 (rAb) at 1 µg/mL, followed by Multi-rAb CoraLite® Plus 594-Goat AntiRabbit Recombinant Secondary Antibody (H+L).
AbFlex® CCNA2 rAb antibody tested by ChIP-Seq.
ChIP was performed using ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 25 µg of K562 cells and 2 µg of CCNA2 antibody. ChIP DNA was sequenced on the Illumina NovaSeq and 27 million sequence tags were mapped to identify CCNA2 binding sites. The image shows binding across a region of chromosome 7.
Background
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
AbFlex® CCNA2 (Cyclin A2) antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells.
CCNA2 (Cyclin A2, also known as CCN1, CCNA) belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. CCNA2 controls both the G1/S and the G2/M transition phases of the cell cycle through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AbFlex® CCNA2 (Cyclin A2) antibody (rAb).
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt. This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
