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AbFlex® CCNB1 (Cyclin B1) antibody (rAb)
| Aliases: CCNB, G2/Mitotic-Specific Cyclin-B1 |
| Catalog No: 91523 | Format: 100 µg | $400 | Add to Cart |
| Catalog No: 91524 | Format: 10 µg | $90 | Add to Cart |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.2 - 1 µg/mL
IP: 2 µg per IP
IF: 0.4 - 2 µg/ml
Immunogen
This CCNB1 (Cyclin B1) antibody was raised against a peptide of human CCNB1 (Cyclin B1).
Buffer
Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
AbFlex® CCNB1 (Cyclin B1) antibody (rAb) tested by Western blot.
40 µg of BEWO (Lane1) and HeLa (Lane2) whole cell extracts were probed with AbFlex® CCNB1 (Cyclin B1) antibody at 0.5 µg/mL.
AbFlex® CCNB1 rAb antibody tested by immunoprecipitation.
2 µg of antibody was used to immunoprecipitate CCNB1 from 1 mg of BEWO whole cell extract, 40% of IP product (Lane 3) and 40 µg of Input (Lane 1) proteins were run on an SDS-PAGE and probed with the same antibody at 0.5 µg /ml. Tween-20 at 0.1% was added to blocking buffers to perform Western blot and reduce background. 2 µg of rabbit IgG was used as negative control for IP (Lane 2).
AbFlex® CCNB1 rAb antibody tested by immunofluorescence.
CCNB1 was detected in human CCNB1 cells using AbFlex® CCNB1 antibody at 2 µg/mL. Followed by Multi-rAb CoraLite® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L).
Background
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.
AbFlex® CCNB1 (Cyclin B1) antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells.
CCNB1 (G2/mitotic-specific cyclin-B1), also known as Cyclin B1, is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M (mitosis) transition.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt. This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
- WB = Western Blotting
