AbFlex® c-Myc antibody (rAb)

Aliases: MRTL, MYCC, c-Myc, bHLHe39
Catalog No: 91539 Format: 100 µg $400 Add to Cart
Catalog No: 91540 Format: 10 µg $90 Add to Cart

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Antibody Type:
Recombinant
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
50.6 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunofluorescence Validated

Application Notes


Validated Applications:
WB: 0.1-1 µg/ml each
IF: 0.4-2 µg/ml

Immunogen

This c-Myc antibody was raised against a peptide of human c-Myc

Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 40% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 
AbFlex<sup>®</sup> c-Myc antibody (rAb) tested by Western blot.

AbFlex® c-Myc rAb antibody tested by Western Blot.
40 µg of Raji whole cell extracts were run on SDS-PAGE and probed with AbFlex c-Myc antibody at 0.1 µg/mL.

AbFlex<sup>®</sup> c-Myc antibody (rAb) tested by immunofluorescence.

AbFlex® c-Myc rAb antibody tested by immunofluorescence.
c-Myc was detected in human Raji cells using AbFlex c-Myc (rAb) at 1 µg/mL, followed by Multi-rAb CoraLite® Plus 594-Goat AntiRabbit Recombinant Secondary Antibody (H+L).

Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

AbFlex® c-Myc antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells.

MYC (Myc proto-oncogene protein, also know as c-Myc, MRTL, MYCC, bHLHe39) is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins are associated with thousands of genes, especially those involved in cell growth and proliferation. c-Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • TIP-ChIP = Tagmented, Indexed, and Pooled Chromatin Immunoprecipitation;
  • WB = Western Blotting