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Recombinant c-Jun protein
| Aliases: Jun Proto-Oncogene, AP-1 Transcription Factor Subunit, P39, V-Jun Avian Sarcoma Virus 17 Oncogene Homolog |
| Catalog No: 31116 | Format: 5 µg | $485 | Buy |
Expressed In: Baculovirus Protein Species: Human
Contents
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Background
c-Jun is a component of the heteromeric AP-1 transcription factor complex. AP-1 proteins play a role in the expression of many genes involved in the regulation of cellular processes such as differentiation, proliferation and apoptosis. The transcription factor AP-1 is composed of a mixture of heterodimeric protein complexes derived from the Fos and Jun families, including c-Fos, FosB, Fra-1, c-Jun, JunB and JunD. c-Jun can be activated by phosphorylation from the JNK pathway.
Application Notes
Recombinant c-Jun is suitable for DNA and protein-protein interaction assays. 100 ng is sufficient for DNA-protein and protein-protein interaction studies. The molecular weight of the protein is ~43 kDa. The standard curve for TransAM® AP-1 c-Jun was generated using the range of 320-40 ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Protein Details
Recombinant c-Jun was expressed from full length (accession number NM 002228) with an N-terminal His-Tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
TransAM® standard curve generated using Recombinant c-Jun protein.
The standard curve for TransAM® was generated using a range of 320-40 ng of protein and run on the TransAM® AP-1 c-Jun ELISA Kit.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
