Recombinant DNMT1 protein

Aliases: AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN
Catalog No: 31404 Format: 20 µg $405 Buy
Catalog No: 31800 Format: 1 mg $3,800 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Full length recombinant DNMT1 protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 1mM DTT and 0.2 mg/ml 3 × Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

Background

DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).

DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

Application Notes

Recombinant DNMT1 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

DNMT Activity Assay Conditions: Assay was performed using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C.

Active Motif has a collection of small-molecule epigenetic modulators that target DNMTs - click here to view the list.

Protein Details

Full length recombinant DNMT1 protein (accession number NP_001370.1) was expressed in Sf9 cells and contains an N-terminal 6×His-Tag and FLAG-Tag with a molecular weight of 188.2 kDa. The recombinant protein is >90% pure by SDS-PAGE.

References


This product was used in the following publications:
Cell Stem Cell. (2018). Nov. 21: pii: S1934-5909(18)30541-1. PMID: 30554961.

 
DNMT1 protein Coomassie gel

Recombinant DNMT1 protein gel.
Recombinant DNMT1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

DNMT1 protein DNMT assay

Recombinant DNMT1 protein activity assay
Recombinant DNMT1 protein activity was measured using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.