DNMT1 antibody (pAb)

RRID: AB_2793389
Aliases: AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN
Catalog No: 39905 Format: 100 µg $445 Buy
Catalog No: 39906 Format: 10 µg $110 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
185 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated

Application Notes

Applications Validated by Active Motif:
IP: 10 µg per IP
WB*: 0.5 - 2 µg/ml dilution
IF: 1:500 dilution

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

For DNMT1, we also offer AbFlex® DNMT1 Recombinant Antibody (rAb). For details, see Catalog No. 91175.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This DNMT1 antibody was raised against a recombinant protein corresponding to amino acids 1-110 of human DNMT1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

DNMT1 antibody (pAb) tested by Immunoprecipitation.
10 µg of DNMT1 antibody was used to immunoprecipitate DNMT1 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µg of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the DNMT1 antibody at a dilution of 0.5 µg/ml.

DNMT1 antibody (pAb) tested by Western blot.
Nuclear extract of HeLa cells (20 µg per lane) probed with DNMT1 antibody (pAb) at a dilution of 0.5 µg/ml.

Detection of DNMT1 by immunofluorescence.
U2OS cells were stained with DNMT1 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: DNMT1 antibody staining. Right panel: merge.

Background

DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).

DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for DNMT1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting