Recombinant BRDT (257-382), GST-Tag

Aliases: CT9, BRD6
Catalog No: 31483 Format: 100 µg $410 Buy
Catalog No: 31883 Format: 1 mg $2,900 Buy
Expressed In: E. coli Protein Species: Human

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Contents

Recombinant BRDT (257-382), GST-Tag protein expressed in E. coli and supplied in 25 mM Tris pH 7.4, 150 mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

Background

BRDT is similar to the RING3 protein family. It possesses two bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

The peptide corresponding to amino acids 257-382 that contains the second bromodomain sequences of BRDT (accession number NP_997072.1) was expressed in E. coli and contains an N-terminal GST-Tag with an observed molecular weight of 42.8 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 
BRDT (257-382), GST-Tag protein gel.

Recombinant BRDT (257-382), GST-Tag protein gel.
BRDT (257-382), GST-Tag protein was run on an SDS-PAGE gel and stained with Coomassie blue.

BRDT (257-382) HTRF activity assay

Recombinant BRDT (257-382) HTRF activity assay.
3.3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.

BRDT (257-382) HTRF activity assay

Recombinant BRDT (257-382) HTRF activity assay.
3.3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (257-382) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.