MAD2L1 antibody (mAb)

RRID: AB_2793241
Clone: 17D10
Aliases: MAD2, HSMAD2
Catalog No: 39399 Format: 200 µg $320 Buy
Catalog No: 39400 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
23 kDa
Reactivity:
Human, Mouse, Wide Range Predicted

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 2 - 5 µg/ml dilution

Immunogen

This MAD2L1 antibody was raised against full-length recombinant human MAD2L1.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

MAD2L1 mAb (Clone 17D10) tested by Western blot.
MAD2L1 detection by Western blot. The analysis was performed using 20 µg HEK293 whole-cell extract and MAD2L1 mAb (Clone 17D10) at a 2 µg/ml dilution.

Background

MAD2L1 (aka HSMAD2, MAD2, MAD2-like 1) is the human homolog of the budding yeast MAD2, or mitotic arrest deficient 2 gene. MAD2L1 is a component of the mitotic spindle assembly checkpoint, which monitors the attachment of the mitotic spindle to the kinetochores of segregating chromosomes and delays the onset of anaphase until attachment is complete and the chromosomes are properly aligned on the metaphase plate. MAD2L1 associates only with unattached kinetochores in prometaphase, and prevents cell cycle progression by associating with the Anaphase Promoting Complex (APC), specifically restricting the activity of the CDC20 protein.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for MAD2L1 antibody (Clone 17D10).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting