CENP-E antibody (mAb)

Clone: 1H12
Aliases: KIF10, CENPE, PPP1R61
Catalog No: 39619 Format: 200 µg $435 Buy
Catalog No: 39620 Format: 10 µg $105 Buy

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Antibody Type:
Protein G Chromatography
Molecular Weight:
320 kDa
Human, Mouse


Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Validated Applications:
ICC/IF: 1 µg/ml dilution

This CENP-E antibody can be used in Western blot to detect CENP-E if the protein has been first enriched by immunoprecipitation.


This CENP-E antibody was raised against full-length human CENP-E protein.


Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.


CENP-E antibody (mAb) tested by immunofluorescence.

CENP-E mouse mAb (Clone 1H12) tested by immunofluorescence.
Staining of HeLa cells with CENP-E mouse mAb (Clone 1H12) at a 1 µg/ml dilution (red) and DAPI (blue).

CENP-E antibody (mAb) tested by immunofluorescence.

Detection of CENP-E by immunofluorescence.
U2OS cells were stained with CENP-E antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: CENP-E antibody staining. Right panel: merge.


Centrosome-associated protein E (CENP-E) is a kinesin-like protein and an integral component of kinetochore fibers that link centromeres to spindle microtubules. CENP-E localizes to kinetochores throughout all phases of mitotic chromosome movement. After the onset of anaphase it is released, and by telophase becomes bound to interzonal microtubules of the mitotic spindle. It is likely that CENP-E serves as a chromosome motor during anaphase.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CENP-E antibody (Clone 1H12).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CENP-E antibody (mAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting