INCENP antibody (mAb)

Clone: 58-217-2
Aliases: Inner Centromere Protein
Catalog No: 39259 Format: 200 µg $435 Buy
Catalog No: 39260 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein A Chromatography
Host:
Mouse
Molecular Weight:
140 kDa
Reactivity:
Human, Mouse, Wide Range Predicted

Applications

Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:500 - 1:1,000 dilution

Published Applications: see references

Immunogen

This INCENP antibody was raised against a recombinant protein corresponding to amino acid residues 231-398 of human INCENP.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
INCENP antibody (mAb) tested by immunofluorescence.

INCENP mAb tested by immunofluorescence.
Top left: HeLa cells stained with INCENP monoclonal antibody at a 1:1,000 dilution. Top right: Same cells stained with alpha Tubulin mAb. Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.

Background

INCENP (Inner Centromere Protein) is part of the chromosomal passenger complex that associates with chromosomes and regulates mitosis and meiosis. INCENP forms a scaffold holding the CP complex together and plays a role in the regulation of Aurora B and Aurora C kinase activity.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for INCENP antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with INCENP antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting