PARP-1 N-terminal antibody (pAb)

Catalog No: 61639 Format: 100 µg $435 Buy
Catalog No: 61640 Format: 10 µg $105 Buy

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Antibody Type:
Protein A Chromatography
Molecular Weight:
120 kDa


ChIP Validated ChIP-Seq Validated Western Blot Validated Immunoprecipitation Validated

Application Notes

Validated Applications:
ChIP-Seq: 5 µg per ChIP
IP: 10 µg per IP
WB: 0.2 - 1 µg/ml dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This antibody was raised against a His-Tagged fusion protein corresponding to the N-terminal half of human PARP-1.


Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a sera version (Catalog No. 39559) of this antibody is also available.


PARP-1 N-terminal antibody (pAb) tested by ChIP-Seq.

PARP-1 N-terminal antibody (pAb) antibody (rAb) tested by ChIP-Seq.
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from human NCI-H209 lung cancer cells and 5 µg of PARP-1 N-terminal antibody (pAb). ChIP DNA was sequenced on the Illumina NextSeq and 7.7 million sequence tags were mapped to identify PARP binding sites on chromosome 2.

PARP-1 N-terminal antibody (pAb) tested by immunoprecipitation.

PARP-1 N-terminal antibody (pAb) tested by Immunoprecipitation.
10 µg of PARP-1 antibody was used to immunoprecipitate PARP-1 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µg of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the PARP-1 antibody at a dilution of 1 µg/ml.

PARP-1 N-terminal antibody (pAb) tested by Western blot.

PARP-1 N-terminal antibody (pAb) tested by Western blot.
HeLa nuclear extract (25 µg) was probed with PARP-1 N-terminal antibody at a dilution of 1 µg/ml.


PARP-1 N-terminal (ADPRT) encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation and tumor transformation. It also plays a role in the regulation of the molecular events involved in the recovery of cells from DNA damage. Cleavage of PARP-1 (ADPRT) occurs following caspase activation during apoptosis.

For additional information on PARP-1, please see the review article PARP-1: An Abundant and Ubiquitous Protein with Roles in Many Cellular Processes in the Targets & Applications section of our website.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with PARP-1 N-terminal antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting