FOXO1/FKHR antibody (pAb)

Aliases: FKH1, FOXO1A
Catalog No: 39629 Format: 200 µl $435 Buy
Catalog No: 39630 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
90 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:1,000 dilution

Immunogen

This FOXO1/FKHR antibody was raised against a peptide from the C-terminal region of human FOXO1/FKHR.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
FOXO1/FKHR antibody (pAb) tested by immunoprecipitation.

FOXO1/FKHR antibody (pAb) tested by Immunoprecipitation.
10 µl of FOXO1/FKHR antibody was used to immunoprecipitate FOXO1/FKHR from 500 µg of K562 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FOXO1/FKHR antibody at a dilution of 1:500.

FOXO1/FKHR antibody (pAb) tested by Western blot.

FOXO1/FKHR antibody (pAb) tested by Western blot. U2OS nuclear extract (20 µg per lane) was probed with 39629 at a 1:500 dilution.

Background

FOXO1/FKHR (forkhead box 01) belongs to the forkhead family of transcription factors that contain a distinct forkhead domain. Chromosomal aberrations involving FOXO1, such as translocation with PAX3, are associated with aveolar rhabdomyosarcoma. The specific function of this gene is undetermined.

Positive Control

Nuclear extracts from U2OS cells can be used as a positive control for FOXO1/FKHR antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting