SIP1 antibody (pAb)

RRID: AB_2793511
Aliases: ZEB2, SIP-1, ZFHX1B, HSPC082, SMADIP1
Catalog No: 61113 Format: 100 µl $500 Buy
Catalog No: 61114 Format: 10 µl $125 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
180 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB*: 1:500 - 1:1,000 dilution

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Immunogen

This SIP1 antibody was raised against a peptide from the N-terminus of human SIP1.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Western blot of SIP1 antibody.
Nuclear extract of Saos-2 cells (20 µg per lane) probed with SIP1 antibody (pAb) at a dilution of 1:500.

Background

SIP1 (Smad-interacting protein 1, aka ZEB2, Zinc finger E-box-binding homeobox 2) is a sequence-specific DNA binding protein and transcriptional repressor that is involved in normal embryonic development of neural structures and the neural crest. SIP1 belongs to the delta-EF1/Zfh1 family of 2-handed zinc finger/homeodomain proteins and contains a SMAD-binding domain, a homeodomain and two clusters of zinc fingers on the N- and C-termini. SIP1 interacts directly with activated forms of SMAD1, SMAD2 and SMAD3, as well as CBX4 and CTBP1. Mutations in the SIP1 gene cause a form of Hirschsprung disease (HSCR).

Positive Control

Active Motif's Saos-2 nuclear extract (Catalog No. 36025) can be used as a positive control for SIP1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting